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Most clinical evidence for biotin is for oral supplementation, not topical application

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[DOI] [PMC free article] [PubMed] [Google Scholar] Ben-Ari Y, Khalilov I, Kahle KT, Cherubini E (2012) The GABA excitatory/inhibitory shift in brain maturation and neurological disorders

The peptide works quickly for surface-level improvements, and shorter bursts maintain effectiveness without prolonged exposure